Novel immune-related genes in the tumor microenvironment with prognostic value in breast cancer

Abstract Background Breast cancer is one of the most frequently diagnosed cancers among women worldwide. Alterations in tumor microenvironment (TME) have been increasingly recognized as key development and progression breast recent years. To deeply comprehend gene expression profiling TME identify immunological targets, well determine relationship between different prognoses highly critical. Methods The stromal/immune scores patients from Cancer Genome Atlas (TCGA) were employed to comprehensively evaluate TME. Then, characteristics assessed, overlapping genes top 3 Gene Ontology (GO) terms upregulated differentially expressed (DEGs) analyzed. Finally, through combined analyses overall survival, time-dependent receiver operating characteristic (ROC), protein-protein interaction (PPI) network, novel immune related with good prognosis screened validated both TCGA GEO database. Results Although did not correlate stages cancer, it was closely associated subtypes mutations (CDH1, TP53 PTEN), had characteristics. Based on GO functional enrichment analysis, high vs low score groups mainly involved T cell activation, external side plasma membrane, receptor ligand activity. DEGs exhibited better cancer; 15 them especially CD226 KLRC4-KLRK1. Conclusions High KLRC4-KLRK1 levels identified survival specific or cancer. CD226, other new targets seem be promising avenues for promoting antitumor targeted immunotherapy